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Establishment of peritoneal liquid electrophoretogram from healthy horses and horses submitted to experimentally induced intestinal obstruction Arq. Bras. Med. Vet. Zootec.
Nogueira,A.F.S.; Di Filippo,P.A.; Anai,L.A.; Vieira,M.C.; Simplício,K.M.M.G.; Santana,A.E..
The initial inflammatory stages of the colic syndrome include changes known as acute phase response. The aim of this study was to contribute with the establishment of reference values concerning the electrophoretogram of peritoneal liquid from healthy horses and horses submitted to experimentally induced intestinal obstruction. Twenty-one horses were allotted in four groups: duodenal obstruction (DG), ileum obstruction (IG), left-dorsal colon obstruction (MG), and control group (CG). Peritoneal liquid was sampled before obtruction (T0), with 3 hours of obstruction (T3) and 6, 30, 102 and 174 hours after desobstructing (T6, T30, T102 and T174, respectively). Total protein levels were determined by the biuret method and protein fractions were obtained by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peritoneal fluid; Equine; Colic; Acute phase proteins.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000300665
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In vitro biological screening of the anticholinesterase and antiproliferative activities of medicinal plants belonging to Annonaceae BJMBR
Formagio,A.S.N.; Vieira,M.C.; Volobuff,C.R.F.; Silva,M.S.; Matos,A.I.; Cardoso,C.A.L.; Foglio,M.A.; Carvalho,J.E..
The aim of this research was to investigate the antiproliferative and anticholinesterase activities of 11 extracts from 5 Annonaceae species in vitro. Antiproliferative activity was assessed using 10 human cancer cell lines. Thin-layer chromatography and a microplate assay were used to screen the extracts for acetylcholinesterase (AchE) inhibitors using Ellman's reagent. The chemical compositions of the active extracts were investigated using high performance liquid chromatography. Eleven extracts obtained from five Annonaceae plant species were active and were particularly effective against the UA251, NCI-470 lung, HT-29, NCI/ADR, and K-562 cell lines with growth inhibition (GI50) values of 0.04-0.06, 0.02-0.50, 0.01-0.12, 0.10-0.27, and 0.02-0.04 µg/mL,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Annona; Duguetia; Antiproliferative; Anticholinesterase activity.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400308
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